7^th International Conference on Predictive, Preventive and Personalized Medicine & Molecular Diagnostics Oct 05-06, 2017 Chicago, Illinois, USA

Biography:

Abstract:

Since the first successful kidney transplant between the Herrick twins in 1954, the lives of thousands of patients have been saved and transformed through kidney transplantation.  While innovation has continued in kidney transplantation, long term outcomes have not changed.  In the United States, at 10 years post-transplant , 50% of kidney transplant recipients have lost their transplanted kidney.  The lack of change in long term outcomes is partially caused by the increased occurrence  of cancer, infections, and metabolic syndrome found among kidney transplant recipients compared to the general population.  The increases in these post-transplant diseases is caused by the organ rejection medications that recipeints take for life.  Beyond the impact on post-transplant mortality, the medications also dimish quality of life.  The current post-transplant monitoring test is serum creatinine function.  This test is limited in its ability to determine the appropriate immunosuprressant dose,  and determine whether the patient is either under immunosuppressed or over immunosuprssed.  Besides the lack of specificity, the serum creatine test lacks the ability to predict future events such as acute and chronic rejection.  Essentially, transplant nephroogists are force to practice reactive medcine rather than procactice medicine.  There is a need for a post-transplant monitoring test that provides specific and accurate information that will enable nephrologists to prescribe the appropriate dose which will reduce post-transplant cancer, infections, improve qulaity of etc. and prevent the development of acute and chronic rejection.

Biography:

Feyrer-Melk has developed a unique and practical background in Lifestyle Medicine, allowing him to effectively leverage technology and behavioral science to enhance personalized care. His extensive work with healthcare professionals and patients is grounded in proven health and lifestyle medicine principles, making him a thought-leader in the field. Dr. Feyrer-Melk serves as the  Director of Lifestyle Medicine at the Optimal Heart Attack & Stroke Prevention Center, the Chief Science Officer for a Med-Tech company (Nudge, LLC)  and is a valued consultant and speaker.  He received his Ph.D. in Exercise Science & Wellness at Arizona State University under the direct guidance of Dr. Charles Corbin, and his M.Ed. in Human Performance at Bowling Green State University under Dr. Richard Bowers. 

Abstract:

Biography:

Sergey Suchkov, MD, PhD. Professor in Immunology & Medicine. Born 11 January 1957, Astrakhan, Russia; two sons. Education: MD, Astrakhan State Medical University, Russia, 1980; PhD, I.M.Sechenov First Moscow State Medical University, 1985; Doctor Degree, National Institute of Immunology, Russia, 2001. Positions held: Post Doc Research Associate, Institute of Medical Enzymology; Head of the Lab of Immunology, Helmholtz Eye Research Institute in Moscow; Trainee, Laboratory of Immunology, NEI, Bethesda, MD, USA; Chair,, Department for Clinical Immunology, Moscow Clinical Research Institute; Executive Secretary-in-Chief of the Editorial Board, Biomedical Science International journal; Chairman & Director of the Department of Personalized Medicine. Honours: Secretary General, United Cultural Convention, Cambridge, UK. Memberships: New York Academy of Sciences, USA; EPMA, Brussels

Abstract:

A new systems approach to disease to pay its crucial attention on the trend would result in a new branch in the healthcare services, namely, predictive, preventive and personalized medicine (PPPM). The latter is defined as: “the capacity to predict disease development and influence decisions about lifestyle choices or to tailor medical practice to an individual…”To achieve the practical implementation of PPPM concept, it is necessary to create a fundamentally new strategy based upon the subclinical recognition of biomarkers of hidden abnormalities long before the disease clinically manifests itself. This strategy would give a real opportunity to secure preventive measures whose personalization could have a significant influence on demographics!The key benefits of PPPM would include: (i) to detect disease at a subclinical stage; (ii) to stratify patients into groups that enable the selection of optimal preventive treatment; (iii) to reduce adverse drug effects by more effective early assessment of individual drug responses; (iv) to improve the selection of new targets for drug discovery; (v) to shift the emphasis from illness to wellness.PPPM consists of a wide variety of tests and tools. The decision to utilize them or not is made at the microlevel – depending on the type of intervention it could be ordered by administration of hospitals and infirmaries, laboratories or clinics, by doctors, and sometimes directly by individuals/patients! Coordinated measures to optimize the progress should be well-focused on solving the accumulating problems in healthcare and the concomitant economic burden that societies across the globe are facing more and more. PPPM offers great and real promise for the future, and next generations will speak about the XXI century as a time, when healthcare services became predictive and preventive, and its outcomes – secured and guaranteed!

Biography:

Vincent Gallicchio received his PhD in hematology from New York University and his diploma in medicine from the University of Arad, Romania. He performed postdoctoral studies at the University of Connecticut Medical Center and conducted his fellowship in hematology from the Memorial Sloan Kettering Cancer Center. He has published more than 150 research articles in peer-reviewed journals on topics focused on stem cells and hematology, seven books, many book chapters, and has been awarded eleven United States and one international patents for developmental therapeutics for AIDS and cancer. He currently serves as director of stem cell therapy for out patient clinical use.

Abstract:

Personalized medicine encompasses many different components one of which is Regenerative Medicine. Regenerative medicine is a branch of translational research in tissue engineering and molecular biology, which deals with the process of replacing, engineering or regenerating human cells, tissues or organs to restore or establish normal function. This field holds the promise of engineering damaged tissues and organs by stimulating the body's own repair mechanisms to functionally heal previously irreparable tissues or organs. Regenerative medicine also includes the possibility of growing tissues and organs in the laboratory and implanting them when the body cannot heal itself. If a regenerated organ's cells would be derived from the patient's own tissue or cells, this would potentially solve the problem of the shortage of organs available for donation, and the problem of organ transplant rejection. Many of the biomedical approaches within the field of regenerative Medicine may involve the use of stem cells. Examples include the injection of stem cells or progenitor cells obtained through directed differentiation (cell therapies); the induction of regeneration by biologically active molecules administered alone or as a secretion by infused cells (immunomodulation therapy); and transplantation of in vitro grown organs and tissues (tissue engineering). Though use of cord blood beyond blood and immunological disorders is speculative, research has been done in other areas. Any such potential beyond blood and immunological use is limited by the fact that cord cells are hematopoietic stem cells (which can differentiate only into blood cells), and are not pluripotential stem cells, as embryonic stem cells, which can differentiate into any type of tissue, they can be induced to “dedifferentiate” as induced pluripotential stem cells. Cord blood has been studied as a treatment for diabetes. However, apart from blood disorders, the use of cord blood for other diseases is not a routine clinical modality and remains a major challenge for the stem cell community. Along with amniotic tissue, adipose tissue, chord blood, chord lining, and Wharton’s jelly, have been explored as sources of mesenchymal stem cells (MSC) in order to treat conditions mediated as a function of aging and inflammation. MSC have been studied in vitro, in animal models, and in early stage clinical trials for cardiovascular diseases, as well as in osteoarthritic conditions, neurological deficits, liver diseases, immune system diseases, diabetes, lung injury, kidney injury, and leukemia. This presentation will review the use of MSCs in these clinical indications.

Biography:

Rosenbloom MD, MBA is the founder and CEO of PEPID LLC, recognized globally as the top developer and electronic provider of clinical decision support. His model of electronic delivery, streamlined concise medical information, built-in workflow and personal creation of thousands of point-of-care support tools is now used in schools, hospitals and institutions in 159 countries worldwide. He is the Medical Director of LIFEFORCE Medical Institute in Chicago, Illinois, providing cutting-edge age management, personalized medicine and optimal performance diagnosis and treatment.

Rosenbloom’s education includes McGill University, Stanford University and Northwestern University’s Feinberg School of Medicine.  He was the recipient of the Dean’s AOA Research Award and the Sigmund S. Winton Award in Biochemistry. He has served as Editor-in-Chief of “Your Health Magazine” and has published articles on drug interactions, age management, low testosterone for men, BHRT, medical errors, and vitamin toxicity. He practiced at Northwestern Memorial Hospital achieving the rank of Adjunct Associate Professor of Medicine.

Among his additional accomplishments, Rosenbloom is the founder and past Chairman of the Unicorn Children's Foundation, an international non-profit organization annually raising millions of dollars and dedicated to promoting early identification and treatment of symptoms associated with ADHD, autism, bipolar, dyslexia and other learning disorders in children and young adults.

Abstract:

With over 50 important pharmacogenomic genes, each with multiple variants, and over 2,000 drugs, the potential drug/gene interactions total in the hundreds of thousands.

It is impractical and virtually impossible to study all potential drug/gene interactions, let alone incorporating drug/drug/gene and drug/gene/gene interactions.

This extensive number of possible drug/gene interactions creates a challenge to implementing pharmacogenomics in practice. Now, through a methodology developed by Dr. Mark Rosenbloom, MD and based on pharmacokinetic and pharmacodynamic principles, clinicians can overcome this challenge by accessing known and predicted drug/gene interactions within seconds from a mobile phone, tablet, or computer.

Dr. Rosenbloom will demonstrate his recently developed drug/gene interactions tool, and a discussion of the methodology used to deliver known and predicted drug/gene, drug/drug/gene, and drug/gene/gene interactions. Designed to provide immediately actionable information to clinicians, the electronic methodology allows for better informed clinical prescribing and patient monitoring decisions.

The methodology is readily adaptable for hospital-wide systems and electronic medical records. The end-result is a clinician, presented with known drug/gene interactions and an array of potential predicted interactions, providing better informed clinical prescribing and patient monitoring decisions. The methodology will be discussed and examples will be given.

Biography:

Jain is a neurosurgeon with career in North America. After retirement from neurosurgery, he started a second career in pharmaceutical medicine and biotechnology in Switzerland. Currently he is a Fellow of the Faculty of Pharmaceutical Medicine of the Royal College of Physicians of UK. Prof. Jain is developing personalized medicine since 1998, and wrote the first monograph on this topic, which evolved into a textbook and the 2^nd edition was published by Springer in 2015. His 465 publications include 27 books (5 as editor and 22 as the author), e.g., “Applications of Biotechnology in Oncology” (Springer, 2014) as the sole author and “Nanooncology: Clinics Lab Med” (Elsevier, 2012). Editorial board memberships of journals include “Technology in Cancer Research & Treatment) and “Nanomedicine”.

Abstract:

Cancer is the most important area for application of personalized medicine, also called precision medicine. This workshop will deal basic principles of personalized oncology. Cancer is a multifactorial complex disease. Variations in behavior of cancer of the same histological type from one patient to another is taken into consideration in addition to variations among patients. Cancer varies both genetically and phenotypically between patients who may have the identical type and stage of cancer. Epigenetic factors also play a role. Each person's cancer is as unique as his or her fingerprint. Personalization of cancer therapies is based on a better understanding of the disease at the molecular level. Molecular diagnostics, molecular imaging, sequencing and monitoring of gene expression by microarrays are important technologies for this purpose. Integration of information gained from various ‘omic’ technologies such as genomics, proteomics, metabolomics, is important in developing personalized approaches. Identification of cancer biomarkers that can enable presymptomatic diagnosis, stratification of cancer, assessment of its progression, evaluation of patient response to therapy, and the identification of recurrences. Study of cancer pathways is providing new targets for anticancer drugs. Among the available therapeutics, matching of the right drug to the right type of cancer and appropriate combination of various options required for a complex disease is important. Improvement of drug formulation and delivery is facilitated by nanobiotechnology. Anticancer medicines can be targeted to the tumor and spare the normal tissues to reduce systemic toxicity.

Biography:

Jack is a retired Insitutional Invesement Research Analyst, previously a career Sales VP in Information Technology who applied his skills in IT, Research and Clinical Trials to build a venture-stage firm recently acquired by ERT Inc. the largest supplier of Clinical Trials Management Software (CTMS) and electronic Patient Reported Outcomes/Clinical Outcomes Assessment (ePRO/COA) He is a popular, gripping, enagaging and fun speaker at leading Life Sciences Conferences around the US. And Europe

Abstract:

Jack Whelan an eight-year survivor of a rare, incurable blood cancer, recently diagnosed with an aggressive metastatic prostate cancer has moved from a career as IT Research Analyst to Research Advocate. Travel with Jack on an intensely personal and humorous bumpy ride on the road to Personalized Medicine still under construction while he is treated for his cancers. Hear his compelling, hopeful and helpful e-Patient observations about how to improve health care using some latest generation Information Technology. Come away with some unique insight that will change your assumptions about research and health IT; get new actionable ideas about how to begin to harness real world data to improve outcomes for patients as we seek safer more effective therapeutics using Advanced Analytics.A tireless Advocate he’s an active with American Association for Cancer Research, received AACR’s 2017 Distinguished Public Service Award, American Society of Clinical Oncology, the NCI’s National Clinical Trials Network, Rare Disease Legislative Advocacy and other professional research and legislative advocacy groups. Jack hopes all of us appreciate the value and noble work of life sciences employees and companies involved in cancer research and care. A decided friend to BioPharma as an engaged, informed e-Patient Opinion Leader (POL) and advocate he regularly speaks to and for these companies, to open much-needed, lines of communications with patients, providers and policymakers.Jack understands the realities of his rare blood and metastatic prostate cancers as he has repeatedly relapsed and has been refractory to many of the therapies received thus far; however his goal is repeated periods of progression free survival during which the promise of science and delivery of successful therapeutics will be achieved. His website is located at Jack-Whelan.com

Biography:

Howard Moskowitz, Harvard PhD in experimental psychology (1969) is founding partner and chief scientific officer of Mind Genomics Advisors. He has published extensively in many fields, using the science of Mind Genomics, which he invented more than 30 years ago. After a distinguished dual career in science and business, he changed course, creating an institute to help younger professionals as well as students and business, benefit from the application of Mind Genomics to practical problems in society, including commerce, health, education, and socal issues. Along with hundreds of scientific papers, he has written/edited 27 published books, with an additional 15 books finished but not yet published.

Abstract:

Mind Genomics, a new science with old roots, reveals what aspects of the topic are important to people, how people differ in the way they respond to these aspects (mind-sets), messages most effective to drive choice and compliance for each mind-set, and finally how to identify the mind-set of a person on a topic, either through personal interaction (the personal viewpoint identifier), or through scored databases (the digital viewpoint identifier). Mind Genomics is a data-driven approach to understand the world of the everyday. A metaphor is ‘cartography of the mind.’ Knowledge is developed in a pointillist style, from the intimate, profound, comprehensive understanding of specific topics, be these situations (e.g., choosing hospital food), regimens (e.g., adhering to the proper schedule for taking one’s drugs), choosing a service, products (e.g., a selecting a hospital in which to give birth), dealing with the ‘customer/patient’ with understanding (e.g., interacting with a teen age patient), etc.. We present the Mind Genomics approach, first in theory, and then in practical applications, showing how messaging can drive potential increases in adherence after discharge from the hospital, as well as better messaging to encourage colonoscopy, and finally messaging to convince the public that one’s hospital is a better place in which to ‘give birth.

Biography:

 Luigi Maselli received the Distinguished Doctor Award at San Raffaele University of Rome in honor of his significant contribution to Lifestyle Medicine in Italy. He is a neuroimmunologist with a PhD in Neurosciences and works as a Research Fellow at Sapienza University of Rome – School of Medicine and Dentistry and as a Visiting Fellow at Gabriele d’Annunzio University of Chieti-Pescara – School of Health Sciences. Dr. Maselli is the founding President of the Italian Lifestyle Medicine Association (ILMA). He is active in patient care, research, speaking, teaching and writing, and wrote the first Italian book on Lifestyle Medicine. After establishing his connection with the Institute of Lifestyle Medicine at Harvard Medical School, where he fell in love with Lifestyle Medicine, he established Lifestyle Medicine programs in several Italian universities. His overwhelming enthusiasm for life and propensity for helping others reflect the values that make Lifestyle Medicine unique- for the treatment and prevention of lifestyle-related chronic diseases.

Abstract:

Converging lines of evidence indicate that stress influences learning and memory processes. Hormones and neurotransmitters released during and after stressful events may hamper memory, executive functions and memory retrieval and it depends on the specific memory process or stage that is affected by stress6 and the activity profile of major physiological stress response systems.Unfortunately stressful events are very common in school daily life. School children often encounter stressful events inside, for example: exams,  evaluations and deadlines, and outside of their school environment and nearly 70% of primary school children report symptoms of stress such as worries, anxiety or sadness. Situations like these could have a critical impact on students performance and on their cognitive ability. Indeed further evidences show that stress may induce a shift from a flexible, ‘cognitive’ form of learning towards rather rigid, ‘habit’-like behaviour. All these stress-induced changes may explain some of the difficulties of learning and remembering under stress in the classroom. Taking these insights from research into account could bear the introduction of supporting strategies to facilitate processes of education for students and teachers as well. Brief mindfulness meditation and lovingkindness meditation are two aspects of mindfulness practice that have the potential to decrease students' feelings of anxiety and stress, and increase their sense of wellbeing and capacity for compassion for self and for others. School appears to be an appropriate setting for such interventions, since children spend a lot of time there and interventions can be brought directly to groups of children in areas of need as part of a preventive approach at little cost. Mindfulness means maintaining a moment-by-moment awareness of our thoughts, feelings, bodily sensations, and surrounding environment. Mindfulness practice may teach students “from the inside out” to cultivate self-management of attention and increase self-awareness by focusing on intrapsychic experiences, such as thoughts, emotional states, the breath, and other bodily sensations.

Biography:

Xiaowu Gai serves as the Director of Bioinformatics in the Center for Personalized Medicine at Children's Hospital Los Angeles. He is an Associate Professor of Clinical Pathology at the Keck School of Medicine at the University of Southern California. Prior to joining CHLA, Dr. Gai was the Director of Bioinformatics and Associate Director of the Ocular Genomics Institute in the Department of Ophthalmology at Harvard Medical School & Massachusetts Eye and Ear Infirmary, Boston. Prior to that, Dr. Gai served as the Director of Biomedical Informatics at Loyola University Chicago Stritch School of Medicine. 

Abstract:

The bioinformatics needs of a large personalized or precision medicine program exemplify the 3Vs of big data challenges: volume, variety and velocity. At the Center for Personalized Medicine at the Children's Hospital Los Angeles, we offer a variety of genomics-based somatic and germline genetic tests (single-gene, gene-panel, whole-exome, whole-genome, chromosomal microarray) for a large number of pediatric patients with either cancers or constitutional disorders. To address the needs and challenges, we implemented a hybrid informatics solution in terms of both the IT infrastructure and the bioinformatics tools. First of all, we employed an on-premise High-Performance Computing Cluster (HPCC) to address the mission-critical computing needs, established the parallel IT infrastucture in AWS cloud for redundancy, bursting nature of our computing needs and cost-effectiveness. Similarly, while implementing a phenotype-driven analytic platform for these genomics-based tests, we used a combination of open-source and custom software tools, striving to provide the most cutting-edge bioinformatics solution at an academic medical center. Examples of our custom solution include 1) a cloud- and web-based variant store using the OpenCGA package that we developed in collaboration with the Genomics England Project team for storing and analyzing somatic and germline variants from whole-exome sequencing and whole-genome sequencing of thousdands of patients, 2) an algorithm for computational phasing two candidate heterozygous variants in a single gene and for improved clinical diagnosis rate of recessive disorders therefore, and 3) an NGS coverage analysis algorithm that is 10 times more efficient than the most popular similar tools, namely SAMtools and Sambamba. 

Biography:

Miglarese joined Caris Life Sciences as Vice President, Research & Development in August 2015.  Prior to joining Caris, Dr. Miglarese founded OncoNex Consulting LLC and served as an advisor and interim Chief Scientific Officer for Cielo Therapeutics.  Before that, he held various positions in the BioPharma sector including Chief Scientific Officer at GeneCentric Diagnostics, Vice President of Oncology Translational and Clinical Science at OSI/Astellas, Assistant Director of Translational Research at Array BioPharma and Section Head in the Department of Cancer Research at Bayer HealthCare Pharmaceuticals.  Dr. Miglarese received his BS in Biology from Virginia Tech and earned his PhD from the Department of Microbiology and Immunology at the University of Virginia.  He continued his post-doctoral training at Pfizer Central Research and in the Department of Dermatology at Yale University School of Medicine.

Abstract:

Despite significant advances in precision medicine in oncology, the performance of predictive biomarkers and companion diagnostics remains profoundly suboptimal.  Clinical benefit from specific cancer therapies is determined by subtle differences in tumor system biology that are inherently difficult to access. Accordingly, highly multiplexed methods are required to evaluate these perturbations.  We developed the ADAPT Biotargeting System^TM which employs aptamer-based polyligand profiling to distinguish a wide range of complex yet subtle phenotypes including response to molecularly targeted therapy.  We show that the ADAPT Biotargeting System^TM can distinguish tumor tissues from breast cancer patients who either did or did not derive benefit from HER2-targeted trastuzumab-based regimens. Test performance was assessed by calculating AUC values from ROC curves and by generating Kaplan-Meier plots using time to next therapy (TTNT) as a surrogate measure of clinical benefit.  ADAPT^TM test-positive patients showed improved clinical outcomes from trastuzumab-based treatment (median TTNT of 429 days versus 129 days for test-negative patients [n=61, HR=0.384, p=0.001]). Interestingly, the ADAPT test also identified patients who received significant clinical benefit from trastuzumab-based therapy despite having HER2-/low scores by IHC (median TTNT of 421 days versus 199 days [n=23, HR=0.119, p=0.001] for chemotherapy alone).  Thus, the ADAPT Biotargeting System^TM has the potential to identify the patients who would benefit from trastuzumab-containing regimens despite standard HER2 testing suggesting otherwise.  We are currently building a number of ADAPT assays to improve upon suboptimal predictive biomarkers currently used for a wide range of cancer therapies.

Biography:

Vincenzo Silani completed his MD from the University of Milan Medical School and he is Professor of Neurology and Chairman of the Department of Neurology and Laboratory of Neuroscience at the same University and at the IRCCS Istituto Auxologico Italiano. Director of the “Dino Ferrari” Center for Neuromuscular and Neurodegnerative Diseases, he has plublished 250 papers in reputed journals. He is Fellow of the European Academy of Neurology (EAN) and has been serving as an editorial board member of different journals. Actively involved for several decades in both clinical and basic research in ALS, he is leading a large academic clinic devoted to the diagnosis and treatment of neurodegenerative diseases such as ALS, FTD, PD, HD, and AD.  

Abstract:

ALS is still a progressive and uniformly fatal neurodegenerative disease: extraordinary progress in understanding the biology of the disease and the number of genetic factors identified provide new reason for optimism that meaningful therapies will be soon applied firstly in patients with a genetic basis. Cases of ALS with C9orf72 mutations are so frequent both in familial and sporadic patients to justify a personalized treatment: the antisense oligonucleotide against SOD1 (another critical gene in familial ALS) delivered intrathecally for patients with SOD1 familial ALS in a phase 1 trial demonstrated no safety or tolerability concerns (Miller et al., 2013). Furthermore, C9orf72 is critical also for Frontotemporal Dementia (FTD), being one of the leading causes of the disease. Precision medicine applied to the ALS/FTD patients carrying a C9orf72 mutation may represent a proof of principle for the extraordinary role of precision medicine in neurodegenerative diseases. Further supporting this application, antisense oligonucleotide are able to reverse pathological features in neurons derived from iPS cells or in fibroblasts obtained from C9ALS/FTD patients, providing an elegant model for a parallel in vitro and in vivo precision medicine. Converging efforts tend to suggest that precision medicine applied to ALS/FTD will soon lead to successful strategies for attenuating the lethal course of both disease. 

Biography:

Fratazzi devised the concept of SCIO and founded the first SCIO –BBCR Consulting in 2009, with the objective of actively contributing to innovation in the clinical process. She acts as a consultant to drug and device companies, and investors. She is a renowned Immunologist; contributed to the registration of 4 drugs. Recipient of 2013, 2014 and 2015 Best Pharmaceutical Consultant, Member of Advisory Board and Board of Directors; Invited speakers and chairman at international conferences. Dr Fratazzi received her early training at the Johns Hopkins University and Harvard University in the USA, and at Imperial College in London.

Abstract:

Precision medicine identifies the evolving field holding the promise to transform our health care system, which consumes almost $3 trillion a year. Clinical trials are at the heart of the drug development process. They represent a significant proportion of the total cost and effectively define the critical path to a regulatory submission. Even the most-simple study costs over $1m and in the later stages of the development phase they can cost hundreds of millions of dollars and take several years to complete. Historically, the clinical trial process had been designed to develop therapeutics for the average patient. This one-size-fits-all approach has demonstrated not effective, as demonstrated by the rising cost and low success rate of clinical trials as well as the percentage of non-responder patients in clinical trials. Precision medicine is the tailoring of medical treatments to the individual characteristics of each patient, and the ability to classify individuals into subpopulations based on their susceptibility to a particular disease or their responses to a specific treatment. The development of precision therapies is closely associated with subpopulations defined by biomarkers. The design of clinical trials for these subpopulations represents a challenge from the perspective of population size, determination of response thresholds and co-development of diagnostic assays to support novel therapies. A fundamental shift in perspective and a willingness to challenge the clinical trial process is required. The SCIO-concept solution provides innovation to the trial process in which new technologies find the ideal background to be developed and applied. 

Biography:

Recognizing that design of molecular interactions of larger molecules often exceeds our present scientific capabilities, Michael’s personal interest lies in the advancement of combinatorial methods at the border line between molecular biology, medicine and chemistry and its exploration for pharmaceutical and medical relevant research. He has been working with enzyme inhibitors as well as membrane and nuclear receptors. Present activities focus on the improvement of peptide phage display methods to establish novel cell type specific ligands and towards a better understanding of the immune response amongst others in allergies and infectious disease.

Michael studied chemistry in Hamburg with a diploma thesis on bioluminescent nucleic acid derivatives in 1985. Three years later he received a doctoral degree at the former German biotechnology center GBF (Braunschweig) for protein design of protease inhibitor hPSTI. After postdoctoral years in protein crystallography and research on melanocortin receptors he served as CSO and CBO for phage display and CRO companies between 1998 and 2009, when he was appointed group leader of a continuously growing group at Fraunhofer IZI in Leipzig. He is now also heading a spin off company in Leipzig for epitope mapping services.

Abstract:

Mapping of antibody epitopes with standard peptide phage display is a common but not always really satisfying approach. Refinement of library parameters for and application of deep sequencing in combination with a novel software analysis tool results in epitope information based on hundreds or more sequences presenting epitope/mimotope variations. The approach does not require more than 2 selection rounds and can be run in a relatively short time. Even when applied to sera we observe significant enrichment of motifs disease related antibody epitopes. These can all be retrieved from a single set of data obtained from a single phage display  experiment with 1-2 µl serum. Due to the statistic approach based on short motifs of 3-4 amino acids structural motifs are also revealed.

This detailed epitope/mimotope information can be used to generate peptides for various applications. This presentation will deal with the immunodiagnostic tools that can be generated from our data. We are able to generate arrays that enable for the first time an individualized analysis of a patient’s immune response even towards individual cross reactive proteins. In the case of allergies it allows us to determine potential cross reactivity to related species, since an individual epitope’s cross reactivity can be predicted from sequence alignments.

The technology is today well enough established to approach larger projects like our present efforts to map most major food allergens or the identification of markers for virus serotypes.

Biography:

Valdes is a tenured Professor of Pathology and Laboratory Medicine at the University of Louisville’s School of Medicine where he has held the appointments of Distinguished University Scholar and Senior Vice-Chairman. Dr. Valdes received his Ph.D. in Molecular Biophysics and completed Postdoctoral Training in Clinical Chemistry and Toxicology at the University of Virginia.  He is nationally and internationally recognized as a leader in advancing the profession of laboratory medicine; has served on Federal regulatory committees; as president of many national professional organizations; and, has received several distinguished scientist and professional recognition awards. Dr. Valdes has authored more than 260 publications, holds several patents and is a pioneer in establishing the application of personalized and precision medicine via laboratory diagnostics.

Abstract:

Polypharmacy, the simultaneous consumption of multiple medications, is not presently considered a health condition such as diabetes, high blood pressure or other chronic health syndromes. The use of multiple medications is growing at an alarming rate with reports documenting a range of 12 to 22 prescriptions being used on average by individuals >50 years of age.  The healthcare consequences range from drug-drug interactions, adverse drug events, prescribing cascades, chronic dependence and hospitalizations, all of which have significant health and economic consequences.  Recent attention on precision medicine and personalizing therapeutics, along with advanced health informatics technology, provides the focus needed to manage this condition and avoid its complications.  First, however, it is important to recognize polypharmacy as a healthcare condition or syndrome and how clinical laboratory diagnostics can play a role in addressing this national problem.  Our discussion centers on the use of laboratory medicine diagnostics in preventing and managing pharmacotherapy with the use of combined pharmacogenetic testing and informatics tools designed to guide selection and dosing of medications.  We urge establishing “polypharmacy” as a recognized healthcare syndrome that, if properly managed based on present technologies, can optimize clinical pharmacotherapy, provide a more precise personalized therapeutics and reduce economic burdens.

Biography:

K Mukhopadhyay has completed her PhD at the age of 27 years from Calcutta University and received postdoctoral training at Tumour Laboratory, Tokyo, Japan. She is the Chief Scientist of Manovikas Biomedical Research and Diagnostic Centre, Kolkata a premier non-government organization in the eastern India for training and rehabilitation of intellectually challenged children. She has published more than 75 articles in reputed journals and had guided 11 graduate students leading to PhD.

Abstract:

Lack of inhibition, poor decision making and scholastic backwardness are major weaknesses observed in Attention Deficit Hyperactivity Disorder (ADHD) probands. Extensive research indicates contribution of neurotransmitters, chiefly dopamine (DA)/ norepinephrine (NE) and hence, medications have been developed targeting these systems. However, due to broad variation in response, treatment is often discontinued. We analyzed role of DA/NE gene variants, DRD4 exon 3 VNTR, rs28363170, rs3785143, rs1611115 and rs4680, in treatment response. ADHD probands diagnosed following the Diagnostic and Statistical Manual for Mental Disorders-IV were evaluated by the Conners’ Parent Rating Scale-revised (CPRS-R) to estimate the behavioral problems, inattention, hyperactivity and ADHD index. Peripheral blood collected from drug naïve ADHD probands, after obtaining informed written consent for participation, was used for genomic DNA isolation and analysis of gene variants. Probands were prescribed either Methylphenidate (MPH) or Atomoxetine (ATX) and post-treatment outcome was assessed using the CPRS-R. This pioneering study on Indian ADHD probands revealed more pronounced side effects after MPH treatment as compared to ATX. Gene variants showed correlation with MPH and ATX induced improvement. Quantitative trait analysis also revealed significant association between rs28363170/rs3785143 variants and response to medication. It may be inferred from the study that rs28363170 and rs3785143 could be major modulators for treatment outcome in this population; while MPH may be more beneficial in presence of rs28363170 10R and rs3785143 T variants, ATX treatment may provide relief in presence of rs28363170 9R and rs3785143 C variants.

Biography:

Madhu Kalia is currently working in the Department of Biochemistry and Molecular Biology, Sydney Kimmel Medical College of Thomas Jefferson University, Philadelphia Pennsylvania, She has published numerous research papers and articles in reputed journals and has various other achievements in the related studies. She has extended her valuable service towards the scientific community with her extensive research work.She is the recipient of numerous awards for her expert research works in related fields. Her research interests reflect in her wide range of publications in various national and international journals

Abstract:

The fundamental goal of molecular diagnostics in oncology is to accurately identify the cancer. Recent trends in genetics, molecular diagnostics and the liquid biopsy are shaping every aspect of oncology today.  Liquid biopsy and related technologies have expanded the study of tumor dynamics and mutational drift over time.  Liquid biopsy provides a useful surrogate for traditional tissue biopsy as an easy, non-invasive access to molecular information about the tumor genome. It helps identify therapeutic targets, follows acquired resistance mechanisms and serves as a diagnostic/screening tool. This presentation will discuss the current status of liquid biopsy, its trends, its limitations and its future potential in oncology. Over the past six years discoveries in molecular diagnostics coupled with developments in the biotechnology of liquid biopsy, have led to enormous progress in the diagnosis and treatment of patients with cancer.  These advances have improved the detection limits of cancer and have greatly improved treatment decisions.  As the spectrum of genes implicated in cancer expands and the technologies used in liquid biopsy grow, there is every reason to expect that there will be more improvement in the diagnosis and treatment of cancer.

Biography:

Manjit S. Bal is MBBS & MD (Pathology) from GMC Amritsar (India), and has a fellowship (FICP) in Pathology; he retired after >37 years of govt. job icluding >31 years as faculty and 17 years as department head; currently, professor Pathology in a pvt. Medical College. Areas of interest: Histopathology, Cytology, Oncopathology; he had trained 115 postgraduates and published 111 papers in Indian & over sea journals; He is an Editorial Board Member and reviewer for reputed journals. He is a regular columnist of health articles in Punjabi for Indian & over sea news-papers; he has authored 11 books in Punjabi, English & Hindi on health topics and literary. Non-medically he has produced two films on his own stories, script, music and lyrics; has a music CD on flute; has traveled widely in India and abroad (USA, Canada, Australia, Singapore etc.) to participate in scientific conferences & as tourist.

Abstract:

Biomarkers are produced by cancer cells or other cells in response to cancer or some other conditions. These are also produced by normal cells, however higher levels appear in malignancy. Most biomarkers are proteins though recently, patterns of gene expression and changes in DNA have also begun to be used as tumor markers. Various biomarkers are in clinical use, some are associated with only one type of cancer, whereas others are associated with two or more cancer types. No “universal” tumor marker has yet been found to detect any kind of cancer. Sometimes, non-cancerous conditions can also cause elevated levels of certain markers, so there are some limitations to their use.
 Cancer of Prostate is the second most common cause of cancer and the sixth leading cause of cancer death among men worldwide (Sartoria & Chanc, 2014). In India also, accoding to reports of four PBCRs (Delhi, Kolkata, Nagpur and Thiruvananthpuram), prostate is the second leading site of cancer
 Prostate-specific antigen (PSA), is elevated in prostate cancer. In 1994, the FDA approved the use of the PSA test in conjunction with digital rectral examination (DRE) to test asymptomatic pateints for prostate cancer. Men with symptoms of prostatism often undergo PSA testing along with digital rectral examination (DRE). In addition to carcinoma prostate, a number of benign conditions can also cause high PSA levels, like prostatitis, prostatic surgery (TUR) and sometimes benign prostatic hyperplasia (BPH).PSA levels increase with the increasing Gleason's grade in prostatic cancer.
 We performed Tru-cut biopsies in 100 suspected cases of carcinoma prostate. 59 biopsies were positive for adenocarcinoma prostate, 35 had benign prostate hyperplasia, one was prostatitis and 5 biopies were inadequate. PSA levels were found high according to their Gleason's grades in ca prostate

Biography:

Sharipov К.О. 1990 year graduated from the Pharmacy Faculty of Almaty State Medical Institute,1994 year completed dissertation of chemical science in Institute of Chemical Science of Kazakhstan Republic,2003 year he completed doctor’s dissertation of biological science and from 2004 year he is full professor. He has published more than 100 articles in reputed journals and presentation of scientific reports on the different international conference and seminars (Russia, France, Germany and China). He is editorial board member 2 journals: “Trace elements in medicine” Moscow, Russia, “Actual problems transport medicine” Odessa, Ukraine.   

Abstract:

Medicine of the XXI century is inseparably linked and more often uses data of omiks technologies for the diagnosis and treatment of different diseases. The using of myltuomics technology in biomedicine contributes to the formation of innovative concept of individual approach to each patient, i.e. personalized medicine. In the development of personalized medicine not only use genomic technologies and genotyping, but other omiks technologies such as metabolome research, methabonome, metallom, etc. Metallom – is a quantitative measurement of metallom components (products of interaction of ionic and atomic forms of metals with endogenous ligands) Nowadays the role of many chemical elements is established in processes of growth, differentiation, regeneration, cell apoptosis and necrosis, as well as in the pathogenesis of some diseases, which is accompanied by significant changes in the element status of body. In our research’s we determined the content of metallom component more than 25 chemical elements including vitally important in people's hair and improved personalized approach to each patient based on identified regularities of quantitative changes in the body. As would be expected elementogramma of examinees people were mostly in the normal range, and some deviations had multidirectional nature. Deviation of essential and trace elements from norm was more specific (for such elements as cobalt, zinc and copper downward and for silicon upward, that might indicate or lead to in the future to certain orphan diseases or disturbing signal comprehensive study and personalized approach).Thus, we can conclude, that a personalized approach to increase the effectiveness of treatment for each patient with using myltuomics technology and provides specific therapeutic effects, reducing the risk of unwanted side effects, precludes mistake assignment ineffective drugs reduces the cost of treatment and develops predictive and preventive medicine. 

Biography:

Skalny has completed his PhD at the age of 28 and D.Sc at the age of 38. He is the vice-president of Trace Element – Institute for UNESCO, head of medical elementology chair of RUDN  University, president of ANO Centre for Biotic Medicine, vise-president of Federation of European Societies on Trace Elements and Minerals, chairman of Russian Society of Trace Elements in Medicine, editor-in-chief «Trace Elements in Medicine» (Russia), member of editorial boards of "Journal of Trace Elements in Medicine and Biology" (Elsevier), and "Biological Trace Element Research" (Springer). He has published more than 400 papers, h-index 27 (RU). 

Abstract:

Based on the database containing more than 500,000 cases from Russia and more than 40 countries since 2000 we have developed a medical technology for detection and correction of impaired mineral homeostasis. The basis of the technology is personalized diagnosis of trace element profile of the organism with subsequent correction using monoelement supplements and efficiency control. In contrast, it has been demonstrated that the use of vitamin-mineral complex has low efficiency due to: i) simultaneous administration of numerous minerals and trace elements, that may result in competition and ultimately, reduced bioavailability of one of the components (e.g. iron, copper, zinc); ii) the absence of personalized approach, as the patients obtain a standard commercial complexes of minerals and vitamins irrespectively of their real requirements; iii) problems with assessment of the vitamin-mineral complex efficiency. Therefore, the developed technology significantly improved correction of trace element status of the organism using the principles of personalized medicine. The current technology was successfully used in treatment of trace element disturbances in patients with neuropsychiatric (e.g., autism spectrum disorders), metabolic (e.g. diabetes) disorders, etc. The technology was also used in prophylaxis in persons with extreme lifestyle (soldiers, elite sportsmen, liquidators of nuclear explosion consequences in Chernobyl). Finally, personalized assessment of trace element status allows to reveal the targets for population health policies. In particular, it has been demonstrated that disturbances of trace element status are associated with demographic parameters and morbidity at the population-wide level, being indicative of the possible ‘macro’ effects of ‘micro’ elements.

Biography:

Feyrer-Melk has developed a unique and practical background in Lifestyle Medicine, allowing him to effectively leverage technology and behavioral science to enhance personalized care. His extensive work with healthcare professionals and patients is grounded in proven health and lifestyle medicine principles, making him a thought-leader in the field. Dr. Feyrer-Melk serves as the  Director of Lifestyle Medicine at the Optimal Heart Attack & Stroke Prevention Center, the Chief Science Officer for a Med-Tech company (Nudge, LLC)  and is a valued consultant and speaker.  He received his Ph.D. in Exercise Science & Wellness at Arizona State University under the direct guidance of Dr. Charles Corbin, and his M.Ed. in Human Performance at Bowling Green State University under Dr. Richard Bowers. 

Abstract:

Modifiable personal lifestyle factors play significant and interconnected roles in predictive, preventive and personalized patient optimal health. Leveraging available cutting-edge Technology and Behavioral science models can maximize outcomes in a A Personalized Care Model.  This session will help medical program developers and practitioners learn how to seamlessly incorporate and effectively use the proper practice-matched technology. The focus will be on understanding behavioral science as a powerful component of personalized care, identifying the best technologies for the practice, wearable technology, wireless health technology, health apps, and practice platform technologies.

Biography:

Jacques S. Beckmann was, from end of 2012 till end of 2016, head of clinical bioinformatics at the SIB Swiss Institute of Bioinformatics. He was previously appointed, from October 2002-Sept 2012, Professor of Human Genetics and Director of the Department of Medical Genetics at the University of Lausanne (UNIL) as well as head of the Medical Genetics Service of the Centre Hospitalier Universitaire Vaudois (CHUV). In these functions, he has been involved in the reconstruction and consolidation of the hospital’s medical genetics service as well as the creation of a new department of Medical Genetics (altogether there were close to 100 people working in these two units).  Previously, he held a chair as Full Professor at the Department of Molecular Genetics at the Weizmann Institute of Science, Rehovot, Israel. Initially trained in molecular genetics he then moved to genetics. In the 1980s, together with Prof. M. Soller from the Hebrew University, they pioneered the use of marker-assisted genetic improvement in plants and animals, focusing on Quantitative Trait Loci (QTLs). His interest shifted in 1990 to human genetics with a move to Paris, where he held successively senior research positions at the CEPH, Généthon (Evry), and finally, was involved, together with Mark Lathrop, in the creation of the Centre National de Génotypage (CNG, Evry), where he served as Deputy-Director. During those years, he contributed significantly to the elaboration of genetic, physical and gene maps of the human genome, as well as to the positional cloning of a number of disease loci involved in muscular dystrophy, diabetes and other diseases. His recent research interests also included genomic disorders as well as the genetic basis of complex traits.  Prof. Beckmann has published close to 400 scientific peer-reviewed articles in molecular genetics, genetics and genomics and with about 32’000 citations. 

Abstract:

Ground-breaking scientific developments in high-resolution, high-throughput data gathering technologies enable cost-effective collection and analysis of huge, disparate datasets on individual health. This is also accompanied by the emergence of clinical bioinformatics tools required for the analyses and interpretation of this wealth of data. These developments have triggered numerous initiatives in precision medicine, a data-driven and currently still, essentially a highly genome-centric initiative, centering on the individual. Proper and effective delivery of precision medicine poses numerous challenges. Foremost, precision medicine needs to be contrasted with the powerful and widely used practice of evidence-based medicine. The latter is informed by meta-analyses or group-centered studies from which mean recommendations are derived. These amount at first approximation to a “one size fits all” approach, whose major limit is that it does not provide adequate solutions for outliers, which for one or another trait we all are. In contrast to evidence-based medicine, one of the strengths of precision medicine lies in the area of individualized management, and this includes outliers.

Here, we argue that to achieve these objectives, it will be necessary to bridge between precision medicine and evidence-based medicine. We will also discuss the challenges and opportunities to bridge this gap thereby achieving clinical utility in precision medicine. First, clinical implementation of precision medicine will require worldwide and responsible data sharing, as well as regularly updated training programs. Second, it will rely on a systemic approach, encompassing among others, all human organs, our complex multicomponent holobiome, i.e., including our virome and microbiome, and our environment. Furthermore, we project that through collection, analyses and sharing of standardized medically relevant data globally, evidence-based precision medicine will shift progressively from therapy to prevention, thus leading eventually to improved, clinician-to-patient communication, citizen-centered healthcare and sustained well-being.

Biography:

Shanrong is Director, Computational Biology and Bioinformatics at Pfizer Inc. More than 20 years of experience in computer science, statistics, genetics and computational biology. Deep scientific knowledge in immunology, autoimmune disease, antibody design and biomarker discovery. Have a demonstrated track of records in scientific initiative, innovation, and leadership, including 4 patents, over 20 peer-reviewed publications, and 20 invited talks at international meetings. A recognized pioneer in the field of RNA-seq, big data analysis and cloud computing. Enthusiastic about using NGS technology, computational approaches and informatics systems to drive drug discovery and biological research.

Abstract:

Due to alternative splicing, over 90% of human genes have multiple transcript isoforms. Isoforms of the same gene can play distinct or even opposite biological rules. For instance, gene TP53 has an important role in oncology and different cancer types show different expression profiles of its transcript isoforms. Therefore, it is tempting to quantify RNA-Seq experiments at transcript level, rather than at the gene level. However, estimating the expression of individual isoform is intrinsically challenging because different isoforms of a gene usually have a high proportion of genomic overlap.

Recently, a number of tools have been developed for RNA-seq isoform quantification, including RSEM, Cufflinks, eXpress, Tigar2, Kallisto, Salmon and Sailfish. We performed a systematic evaluation on those methods using both simulated dataset and UHRR and HBRR, and furthermore investigated the impact of gene/isoform structures on the accuracy of isoform quantification. Besides, the library size and relative abundance of different isoforms also influence the quantification results. We determined why RNA-seq is unable to detect less abundant TP53 transcripts and discussed its implications for the general interpretation of RNA-seq data.

Biography:

Jain is a neurosurgeon with career in North America. After retirement from neurosurgery, he started a second career in pharmaceutical medicine and biotechnology in Switzerland. He is developing personalized medicine since 1998, and wrote the first monograph on this topic, which evolved into a textbook and the 2^nd edition was published by Springer in 2015. His 465 publications include 27 books (5 as editor and 22 as the author). One of the books is Handbook of Nanomedicine (3^rd ed Springer, 2017). Currently he is a Fellow of the Faculty of Pharmaceutical Medicine of the Royal College of Physicians of UK.

Abstract:

Personalized medicine simply means the prescription of specific therapeutics best suited for an individual. It is usually based on pharmacogenetic, pharmacogenomic, transcriptomic, pharmaco-proteomic and pharmacometabolomic information. Other individual variations in patients and environmental factors are also taken into consideration. Concept of personalized medicine is the best way to integrate various biotechnologies and their translation into clinical applications. Advances in nanobiotechnology will facilitate the development of personalized medicine by: (1) Nanodiagnostics will improve the sensitivity and extend the present limits of molecular diagnostics, point-of-care devices, biochips and biosensors; (2) improve discovery of biomarkers; (3) facilitate integration of diagnosis and therapy, which is an important part personalized medicine; and (4) nanomedicines are suitable for targeted delivery to lesions. Important areas of application include oncology, cardiology and neurology. In case of cancer the variation in behavior of cancer of the same histological type from one patient to another is also taken into consideration in addition to variations among patients. Personalization of cancer therapies is based on a better understanding of the disease at the molecular level and nanotechnology will play an important role in this area. Anticancer nanomedicines can be targeted to the tumor and spare the normal tissues to reduce systemic toxicity. Personalized nanomedicines will be more effective and safer than conventional medicines. In conclusion, nanobiotechnology is playing an important role in the development of personalized medicine. 

Biography:

Vladislav S.BaranovBorn in 1940, graduated from the State Medical Institute in Lvov (Ukraina), took postgraduate courses and   received a PhD degree in Saint-Petersburg  (Russia) in 1976 .  The    Chief of laboratory for prenatal diagnosis of inherited and inborn diseases at the Ott’s Institute of Obstetrics, Gynecology & Reproduction. Interested in genetic  and cytogenetic aspects of early  development, gene testing of inherited predisposition to common disorders , personalized predictive medicine, gene therapy.  Professor, Corresponding Member of  Russian Academy of  Sciences,   Honorary Scientist, Chief City Expert in Medical Genetics, , The author and co-author   of 29 books   and over 400 scientific papers.

Abstract:

Predictive medicine (PM) as a kid from natural marriage of Human Genome and Medicine has now converted into   quickly expanded area of medical genetics. Main goal of PM may be attributed   to early  identification of  persons,  predisposed to sever complex diseases  before their  obvious  clinical manifestation and  thus  to their prediction, prevention, diagnostics and  efficient personalized  treatment. Progress of PM runs in parallel to spectacular achievements of human genome studies, advances of molecular technologies and their implementation. Started in 2000 as  Predictive Preventive  Personalized  Medicine    (3P -medicine)  it soon  acquired  its Participatory  trait    and  is  now  treated as 4P-medicine.  After short period of recession in 2009-2012 provoked by   “missing heritability” dilemma time for the   robust recovery of PM comes. New system genetics approach (SGA) paved the way to translational medicine which obvious benefits stems from     the complex analysis    of    common disorders. Brief review of   European PPPM Association program of 30 years   development and    similar Precision Medicine program    recently launched in the USA are presented.     Thus   for  20 years period the  PM  has  passed a  long way from primitive  genetic testing    to  systemic genetic  analysis,   from    Genetic  Pass  suggested as far as   2000  to Electronic Genetic Chart of  Health predicted   in 2015.

Biography:

William G. Kearns earned his doctorate at Eastern Virginia Medical School and completed his advanced training during a three-year fellowship in medical genetics at the McKusick-Nathans Institute of Genetic Medicine of The Johns Hopkins University School of Medicine. Kearns is well known in the field of genetics, particularly preimplantation genetics, and continues to serve on the faculty of Johns Hopkins as a medical geneticist and associate professor. His duties include training fellows and graduate students in genetics and reproductive endocrinology. Widely known nationally and internationally for his expertise in genetics, Dr. Kearns is uniquely qualified in his ability to devise and implement genetic testing strategies in order to offer IVF patients an opportunity to achieve their goal – a healthy baby.  He brings valuable experience to the diagnostic and research laboratory, including 13 years in starting and directing a preimplantation genetic diagnostic laboratory prior to founding AdvaGenix.

Abstract:

With the rapid pace of progress in the fields of biotechnology, genetics, and genomics, molecular genetic profiling may soon become an integral tool for clinicians to guide individualized management of many medical conditions. Personalized medicine (also termed personalized genomics, genomic medicine, or precision medicine) refers to the application of patient-specific profiles, incorporating genetic and genomic data as well as clinical and environmental factors, to assess individual risks and tailor prevention and disease-management strategies. Personalized medicine involves the use of an individual's genetic profile to guide decisions made in regards to the prevention, diagnosis, and treatment of disease. The definition encompasses a broad range of current clinical practices in which genomic results are used to guide patient care. We will discuss the applications of whole exome sequencing and reproductive medicine based upon a family profile for genetic diseases that are lethal or with high morbidity. Trio data will be presented and discussed regarding the use and application of personalized medicine in respect to a family’s desire to have healthy children.

Biography:

Jürgen Karl-Josef Hescheler (born 2 May 1959) is a German physician and stem cell researcher. He is director to the Institute for Neurophysiology and a university professor at the University of Cologne. Prof. Hescheler is one of the most eminent and the most productive stem cell researchers worldwide, averaging more than 1000 citations of his publications per year. Hescheler has been working with embryonic stem cells since the late 80’s. He became the first researcher to accomplish an electrophysiological characterisation of stem cells and was also among the first scientists in Germany obtaining permission to do research on human embryonic stem cells.

Abstract:

 Due to their ability to reproduce the embryonic, neonatal and adult differentiation of all different organotypic cellular phenotypes, pluripotent stem cells represent an ideal tool to study physiological processes of embryogenesis under in vitro conditions as well as to provide the basis of cellular therapeutics, to build up test assay systems for drug discovery or toxicology and to develop novel disease models for companion diagnostics within personalized medicine. In particular, embryonic (ES) and induced pluripotent stem (iPS) cells can reproduce all organotypic electrophysiology, signalling cascades and genes involved in the development (functional genomics). This occurs spontaneously within three-dimensional cell aggregates – embryoid bodies (EBs) – which I developed 25 years ago. To select only one lineage, e.g. the cardiac lineage, and to allow the identification of the transplanted cells, transgenic ES and iPS cells were used. They contained a vector with two cloning sites for EGFP and a puromycin resistance for selection under the alpha-MHC promoter – a technology which allowed an easy transfer to bioreactor systems for large scale production.

We aimed at generating iPS cell-derived cardiomyocytes (CMs) and their molecular and functional characterization in comparison to CMs derived from established ES cells on a transcriptomic and electrophysiological level. To demonstrate the ability of ES cells for regenerative medicine and tissue repair, cardiomyocytes differentiated from ES cells were injected into the cryoinfarcted left ventricular wall of adult wild-type mice. Translation from the laboratory into the clinic is one of the remaining key issues remaining for applied stem cell research.

Within two European consortia, ESNATS and DETECTIVE, under my coordination a battery of toxicity tests was developed using human ES or human iPS lines subjected to different standardised culture protocols. Tests cover embryoid bodies in different developmental stages including early embryonic stages (embryotoxicity), neuronal lineages (neurotoxicity), complemented with test systems for hepatic metabolism and cardiac development (cardio-toxicity). Predictive toxicogenomics and -proteomics markers were identified allowing a better predicitvity of human test systems, to reduce, refine and replace animal tests, to lower testing costs, and to support medium/high throughput testing which will be of relevance for companion diagnostics and personalized medicine. .

Reprogramming of fibroblasts from patients with poly- or monogenetic diseases including LQT3 or CPVT syndrome resulted in generation of iPS cells for disease modelling. This novel approach may also enable patient-specific cell replacement therapies, which appears

to be an indispensable prerequisite for a later use in clinics. iPS cells from patients represent a new diagnostic tool for precision medicine, for better analysis of the pathophysiology and to develop personalised strategies for an optimized therapy.

Biography:

Moonjae Cho  is currently working in the Department of Biotechnology, Jeju National University, Republic of Korea. He has published numerous research papers and articles in reputed journals and has various other achievements in the related studies. He has extended his valuable service towards the scientific community with his extensive research work.

Abstract:

Keratinocyte-fibroblast interactions are critical for skin repair after injury. During the proliferative phase of wound healing, proliferation, migration and differentiation of these cells are the major mechanisms leading to tissue remodeling. We have previously reported that glycitin, a major soy isoflavone, stimulates dermal fibroblast proliferation; and the phytochemical, 4′,6,7-trimethoxyisoflavone (TMF), induces migration of HaCaT keratinocyte cells. We therefore investigated whether these compounds display synergistic effects on skin cells during wound healing in vitro and in vivo. Co-treatment with TMF and glycitin synergistically promotes the proliferation and migration of both keratinocytes and dermal fibroblasts, with a 1:1 ratio of these compounds showing the greatest efficacy in our co-culture system. This keratinocyte-fibroblast interaction occurred via the secretion of TGF-β, and the induction of differentiation and proliferation was confirmed in both indirect and direct co-culture assays. In an excisional and burn wound animal model, mice treated with a 1:1 ratio of TMF and glycitin showed faster wound closure, regeneration and scar reduction than even the positive control drug. These data indicate that two isoflavones, TMF and glycitin, act synergistically to promote wound healing and anti-scarring and could potentially be developed together as a bioactive therapeutic for wound treatment.

Biography:

Maria Voyatzi completed her PhD at the age of 26 years from the Aristotle Uninersity of Thessaloniki, Greece and postdoctoral studies from the Hospital of Dermatological and Venereological Diseases of THESSALONIKI , State Clinic. She has published more than 15 papers in reputed journals and participated in many hellenic, european and world congresses.

Abstract:

Our purpose is to study the efficacy of the Alexandrite laser (755nm) and the fractional laser when treating pigmentary lesions , the efficacy of the NdYAG Laser ( 1064 nm ) when treating telangiectasias and hemangiomas and the efficacy of the NdYAG Laser and fractional laser on rejuvenation.We used the data from our private office from the past five years  (2011-2015). We treated 1000 patients with freckles with Alexandrite laser, 200 patients with melasma with fractional laser, 100 patients with postinflammatory hyperpigmentation with Alexandrite laser and 60 patients  with postinflammatory hyperpigmentation with fractional laser. We also studied the side effects of the therapies. Alexandrite laser had excellent results on freckles, while the combination of chemical peels and fractional laser was satisfactory for the treatment of melasma. Post inflammatory hyperpigmentation had an intermediate response. The recurrence rates were higher in melasma and the side effects were generally minimal. We also treated 500 patients with telengiectasias and 300 patients with hemangiomas with NdYAG Laser. As far as telangiectasias were concerned, the results were much better when  the face was treated . In contrast, the recurrence rates were much higher when the legs were treated. The results were impressive in almost all cases of cherry hemangiomas. The combination of NdYAG Lasers and fractional lasers on a  monthly basis had satisfactory results in rejuvenation.

Biography:

Somi Kim Cho is currently working in the Department of Biotechnology, Jeju National University, Republic of Korea . Somi Kim Cho She has published numerous research papers and articles in reputed journals and has various other achievements in the related studies. She has extended her valuable service towards the scientific community with her extensive research work.

Abstract:

The cysteine-rich angiogenic inducer 61 (CYR61), an extracellular matrix-associated protein, is involved in survival, tumorigenesis, and drug resistance. There is an increasing demand for developing agents that target CYR61. Hence, we study the effects of flavones against CYR61-overexpressing human gastric adenocarcinoma AGS (AGS-cyr61) cells. Quercetin (1) down-regulates CYR61 and concomitantly decreases in the levels of MRP1 (multidrug resistance-associated protein 1) and nuclear factor NF-kappa B (κB) p65 subunit, (2) reverses multidrug resistance, and (3) inhibits colony formation in AGS-cyr61 cells. AGS-cyr61 cells treated with quercetin at sub IC₅₀ over a range of 5-FU or ADR concentrations manifested strong synergistic effects with these two drugs. Our results demonstrate that CYR61 is a potential regulator of ABC transporters and quercetin can be the novel agent that improves the efficacy of anticancer drugs by down-regulating CYR61 and ABC transporters.

Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme which contributes to malignant progression in various cancer. We found that compound D inhibits HDAC6 activity, increases acetylated α-tubulin, reduces the level of β-catenin, and suppresses cell proliferation. Increase of α-tubulin acetylation by compound D resulted in tubulin polymerization, and consequently, induced aberrant mitosis. Moreover, treatment with high concentrations of compound D induces cell death by mitotic catastrophe, whereas low concentration of compound D induces senescence with upregulation of p21 and Rb, and increase in the phosphorylation of mTOR and the β-galactosidase activity. Therefore, compound D can also be considered as a promising new candidate for anti-cancer drug development.

Biography:

Rizvan Abdullaev is currently working in the Kharkiv National Medical University Ukraine. He has published numerous research papers and articles in reputed journals and has various other achievements in the related studies. He has extended his valuable service towards the scientific community with his extensive research work.

Abstract:

The aim of the study was to study the semiotics of the herniated intervertebral disks (IVD) of the cervical spine in older children using ultrasonography (US).

Materials and methods. The study included the results of ultrasound examination of 19 children with degenerative changes of the IVD with violation of the integrity of the fibrous ring (FR) and involvement of the elements of the vertebral canal (VC) in the pathological process. In total, 114 IVD were examined, of which changes were detected in 61 (53.5%) disks. US was conducted at the level of disks C2-C3, C3-C4, C4-C5, C5-C6, C6-C7, C7-Th1. Diagnosis of hernia of the IVD was based on the evaluation of the structure of the pulp nucleus (PN) and FR, registration of the FR rupture, narrowing of the PN and radicular canals.

Results. Herniated discs were detected in children aged 16-18 years. Hernia is diagnosed in 19 (16.7%) disks. The rupture occurred in the posterior part of the discs, the free fragment of the PI ripping the thin sheet of the posterior longitudinal ligament, got into the epidural space, being located next to the veins of the epidural plexus. In 8 (42.1%) cases, hernia was formed at the level of C5-C6, in 6 (31.6%) - at the level of C4-C5, in 3 (15.8%) - at the level of C2-C3 and in 2 ( 10.5%) of cases at the level of C6-C7.

The most frequently recorded paramedian hernia - in 12 (63.2%), then the median - in 5 (26.3%) cases and less often in the posterolateral - in 2 (10.5%) cases. The greatest decrease in the sagittal size of the PN was observed with the median size, and the cross-sectional area with the paramedian hernia 

Conclusions. Ultrasound examination is an alternative method of diagnosing herniated intervertebral cervical discs in older children that allows determining the form and level of localization of the degenerative process.

Biography:

Natalia Malara MD PhD, is a medical oncologist with competence of translational medicine, nanotechnology, toxicology in oncologic and cardiovascular field. She conducts clinical and laboratory investigations focusing her attention on circulating tumor and endothelial cells. He acquired a solid experience in nano-biotechnology techniques with translational approach in medicine collaborating with Bionem group guided by Prof Di Fabrizio. He begin her collaboration with Prof Mollace in July 2009, and since then his research activities are also focused on the project of pre-clinical model finalized to improve pharmacological applications in clinical.

Abstract:

The immunotherapy in cancer is based on the ancient concept of activating an effective immune-mediated reaction direct against tumour cells. How to efficiency activate the immune system in this direction is still a challenge. In fact, despite the rapid increase of knowledge in oncology has contributed to improve immunotherapeutic protocols, some issues remain unresolved. Three key points represent the main challenges causing vaccine-resistance: 1. continuous dynamic changing of cancer tissue determines intrinsic tumour cell alterations; 2. adjustments in the tumour microenvironment; 3. low immune recognition against cancer cells. To learn the molecular changes, that take turns insight tumor during its progression depends on the possibility to analyse cancer cells in real time. Often, the clinicians do not have sources of available tumour cells. In fact, the biopsy of the tumour tissue cannot be repeated many times, for systemic and local complications for the patient . On the other hand, the development of vaccine-resistance depends by the heterogeneity of tumor tissue. The cancer heterogeneity represents a limit in the design and application of immunotherapy adopting specific immunogenic protein. The CTCs represent the cancer cell population released in the blood stream and can be considered like a cellular “summary” of the systemic cancer disease. Recent studies report standardized methodology to collect and short-time in vitro expanded CTCs. The protocol, making available cancer cells without modifying their heterogeneity, provides interesting solutions to overcome the degree of immune tolerance and, on the other hand, to reduce the autoimmune spiral triggered by the cryptic epitopes.

Biography:

   Segundo Mesa Castillo. As Specialist in Neurology, he worked for 10 years in the Institute of Neurology of Havana, Cuba.  He has worked in Electron Microscopic Studies on Schizophrenia for 32 years. He was awarded with the International Price of the Stanley Foundation Award Program and for the Professional Committee to work as a fellowship position in the Laboratory of the Central Nervous System Studies, National Institute of Neurological Diseases and Stroke under Dr. Joseph Gibbs for a period of 6 months, National Institute of Health, Bethesda, Maryland, Washington D.C. USA, June 5, 1990. 

Abstract:

There is increasing evidences that favor the prenatal beginning of schizophrenia. These evidences point toward intra-uterine environmental factors that act specifically during the second pregnancy trimester producing a direct damage of the brain of the fetus. The current available technology doesn't allow observing what is happening at cellular level since the human brain is not exposed  to a direct analysis in that stage of the life in subjects at high risk of developing schizophrenia. Methods. In 1977 we began a direct electron microscopic research of the brain of fetuses at high risk from schizophrenic mothers in order to finding differences at cellular level in relation to controls. Results. In these studies we have observed within the nuclei of neurons the presence of complete and incomplete viral particles that reacted in positive form with antibodies to herpes simplex hominis type I [HSV1] virus, and mitochondria alterations. Conclusion. The importance of these findings can have practical applications in the prevention of the illness keeping in mind its direct relation to the aetiology and physiopathology of schizophrenia. A study of amniotic fluid cells in women at risk of having a schizophrenic offspring is considered. Of being observed the same alterations that those observed previously in the cells of the brain of the studied foetuses, it would intend to these women in risk of having a schizophrenia descendant, previous information of the results, the voluntary medical interruption of the pregnancy or an early anti HSV1 viral treatment as preventive measure of the later development of the illness.

Biography:

Livija Sušić has completed Faculty of Medicine at the age of 24 years from J. J. Strossmayer University, Osijek, Croatia. She is internist and head of specialist-consultation Department in Health Centre Osijek. She is also a Post – Graduate student on J. J. Strossmayer University, Faculty of Medicine. She is at the beginning of the scientific career, published 4 papers till now.  

Abstract:

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited cardiomyopathy commonly transmitted as an autosomal dominant trait, characterized by incomplete penetrance and variable expressivity. So far 13 disease genes have been identified, responsible for approximately 60% of all ARVC cases. The pathological consists of  a dystrophy of right ventricle (RV)  myocardium with fibro-fatty replacement which leads to RV aneurysms dilatation, providing a supstrate for life-treatening arrhythmias. The course of the disease is divided into four main stages: subclinical, overt electrical, RV dysfunctional and  biventricular late stage. Dilatation of the RV and global or focal wall motion abnormalities are supposed to increase the risk of thrombus formation.  It is estimated that annual thromboembolic incidence is 0,5/100 patients, which is extremely rare considering the prevalence of disease being between 1 per 2000 and 1 per 5000 inhabitants. We present the case of 61-year-old female patient with multiple thrombi  in a cavity of RV who had a combination of ARVC and hereditary trombophilia (homozygous mutation on 5G PAI- 1 allele). It is very rare combination and diagnostic challenge for us. Considering that diagnostic protocol went retrograde from rare complication according to cause and that it included anamnesis, laboratory tests, interpretation of ECG and holter ECG monitoring, multimodal  imaging methods ( TTE, TEE, MRI, multislice CT, PET CT, coronarography), surgical procedure with extirpation of multiple RV masses and pathohistological analysis, and molecular markers of inherited thrombophilia, we believe that this is phenomenal example of the application of personalized medicine into practice.
 

Biography:

Seyedeh Afrooz Azimi  has condidated in PhD degree from Mashhad Medical University . She is the Quality  Manager  in  Atieh Hospital in Tehran .She researches on NSCLC Diagnostic biomarkers in Dr. Masih Daneshvari  Hospital ,lung Center of Shahid Beheshti Medical University and she cooperates to published texts in scientific & research center.

Abstract:

Epigenetic  in Lung Cancer:The concepts of personalized medicine can be applied to new and transformative approaches to health care.Epigenetic is based on the dynamics of systems biology and uses predictive tools to evaluate health risks and to design personalized health plans to help patients mitigate risks,prevent disease and to treat it with precision when it occurs. Every person has a unique variation of the human genome.Although most of the variation between individuals has no effect on health, an individual's health stems from genetic variation with behaviors and influences from the environment and Epignetic can also be used to predict a person’s risk for a particular disease and more effectve than one or even several genes.

One of the largest issues is the fear and potential consequences for patients who are predisposed after genetic testing or found to be non-responsive towards certain treatments. This includes the nutrition,life style,job effects on patients due to genetic testing results. The right of family members who do not directly consent is another issue, considering that genetic predispositions and risks are inheritable. The implications for certain ethnic groups and presence of a common allele would also have to be considered.

We received the whole information of patients with NSCLC   In this survey that  NSCLC is Multi factorial disease  and related to Epigenetic .If we want to predict the risks of the disease ,we need to know more about the effects, life style and the personal behaviors  more than genetic and Direct exposed parameters .Maybe in future we could to say there is difference for description of disease based on personal life and Epigenetic.

Biography:

Roman Abdullaev is currently Studying in the Kharkiv National Medical University Ukraine. He has published numerous research papers and articles in reputed journals and has various other achievements in the related studies. He has extended his valuable service towards the scientific community with his extensive research work.

Abstract:

The aim of the study was to study the semiotics of the protrusions of intervertebral disks (IVD) of the lumbar spine in older children using ultrasonography (USG).

Materials and methods. The study included the results of ultrasound examination of 63 children with degenerative changes of the IVD without violating the integrity of the fibrous ring (FR) and involvement of the elements of the vertebral canal (VC) in the pathological process. In total, 315 IVD were examined, of which changes were detected in 72 (22.9%) disks. USG was conducted at the level of disks L2–L3, L3–L4, L4–L5, L5–S1. Diagnosis of protrusion of the disc was based on a change in the structure of the pulpous nucleus, thinning and protrusion of the fibrous ring toward the vertebral canal for more than 2 mm, reducing the anteroposterior size of the vertebral or radicular canals.

Results. Paramedian protrusions occurred in 31 (43.1%) cases, posterolateral protrusions in 18 (25.0%), median - in 15 (20.8%), and circular - in 8 (11.1%) cases, respectively. The protrusion of disks at the level L2–L3 was observed in 3 (4.2%) cases, L3–L4 in 19 (26.4%) cases, L4–L5 in 27 (37.5%), and L5-S1 - in 23 (31.9%) cases, respectively. The greatest decrease in the sagittal size of the VC was observed with the median size, and the cross-sectional area with the paramedian hernia.

Conclusions. Ultrasound examination is an alternative method of diagnosing protrusions intervertebral lumbar discs in older children that allows determining the form and level of localization of the degenerative process.