5^th International conference on Predictive, Preventive and Personalized Medicine & Molecular Diagnostics December 01-02, 2016 Valencia, Spain

Xiangyi He was graduated from School of Stomatology, Beijing University and has worked in Lanzhou University for 28 years. He was a Senior Visiting Scholar in University of Helsinki. He is currently a Member of IADR, FDI and CSC and also a Reviewer of stamotological academic journals both domestic and aboard. His research focuses on oral ecology, oral microbiology, oral medicine and dentistry. He has published more than 80 articles in international academic journals and Chinese academic journals.

Objectives: The purposes of this study were to identify differently expressed microRNA (miRNA) after high-LET carbon ion irradiated human tongue squamous carcinoma Tca-8113 cells and to further study the microRNA roles in tumor radiotherapy.\r\n\r\nMethods: Total RNA was extracted from Tca-8113 cell after high-LET carbon ion irradiation. High throughput sequencing technique (HiSeq) was applied for detecting the differentially expressed genes (DEGs). The DEGs were divided into up-regulated and down-regulated genes. Flow cytometry was used for detecting the cell apoptosis; qRT-PCR was used for verifying the results of HiSeq. Via bioinformatic analysis, the distribution of candidate target genes and the significant enriched GO-term genes were obtained in the Gene Oncology (GO); With KEGG pathway enrichment analysis, the main signal pathways which DEGs take part in were obtained.\r\n\r\nResults: High-LET carbon ion irradiation induced the apoptosis of Tca-8113 cell and a total of 13 microRNAs expression changed. Three up-regulated (miR-40, miR-53, miR-643) and 10 down-regulated microRNAs (miR-278, miR-353, miR-31, miR-375, miR-106, miR-577, etc) were identified in Tca-8113 cells by HiSeq after high-LET carbon ion irradiation. Furthermore, the consequence of qRT-PCR was basically the same as the HiSeq. Via GO function analysis, there were 23 GO-terms in biological process, 18 GO-terms in molecular function and 20 GO-terms in cellular component and so 86 KEGG pathways enriched which DEGs take part in.\r\n\r\nConclusions: Our findings indicated that the high-LET carbon ion irradiation played a key role on microRNA expression of Tca-8113 cells and then these differentially expressed microRNA may become the potential molecular diagnostic biomarkers in tumor radiotherapy

Salim Loudjedi has completed his Surgery studies from universities of Tlemcen and Oran, Algeria. He has practiced General Surgery at Tlemcen Hospital. He is specialized in Hepatobilliary Surgery and his Post doctoral thesis deal with EBM. He has published more than 10 papers

Personalized medicine is currently being developed for individual patient care, especially in oncology. It involves, among other things, advanced science (molecular biology, genetics). On the other hand, Evidence Based Medicine (EBM) is becoming a must in modern medicine. However, it has some limitations, especially in the management of the patient. Our work consists in associating the two paradigms knowing that personalized medicine is one of the pillars of the EBM tripod (best external evidence, individual clinical expertise and patients values & expectations). We conducted a study on the management of gall bladder lithiasis by considering two groups of patients: The first group benefited from EBM approach alone while the second group benefited from EBM in combination with personalized medicine. The comparative study used the PICO criteria (Patient Intervention Comparison Outcome). The results obtained with the second group were better. In conclusion, personalized medicine is a plus value to EBM which can be regarded as the “princeps” paradigm of personalized medicine.\r\n\r\n