Ievgeniia Burlaka
.O. BogomoletsNationalMedicalUniversity, Ukraine
Title: Apoptosis controlling system disturbances in children with nephrotic syndrome
Biography
Biography: Ievgeniia Burlaka
Abstract
Hypoxia-related disorders play an important role in irreversible tissue damage via activation of certain secondary processes. The objective of the current project was to study the levels of cellular hypoxia, apoptosis controlling factors in relation to the value of kidney function impairment and to evaluate possible protective effects of the treatment with antioxidant agent tocopherol in pediatric patients with nephrotic syndrome. An examination ofrenalbiopsies of 53 patients (agedfrom 10 to 15years) withnephrotic syndromewasdone. Conventional clinical investigations, immunogistichemistry, immunoblotting have been applied in this study. We show that nephrotic patients reveal a high level of cellular hypoxia marker HIF-1α and its dependence on the level of kidney function impairment and proteinuria. The progression of the sclerosis as a sign of irreversible kidney damage was accompanied by gradual increase in expression of proapoptotic factor Bax. Administration of the antioxidant tocopherol in addition to the conventional scheme (immunosuppressive agents, ACE inhibitors)resulted in an improvement of hypoxia level, proapoptotic factor Bax expression, clinical symptoms. Thus, hypoxia-induces disorders in nephrotic children presented by disturbances in apoptosis controlling system can be attenuated by the antioxidants application in addition to the conventional treatment.