Biography:

Guilherme Martins Santos completed his Bachelor in Veterinary Medicine in 1997, at the University of Uberlandia, Brazil. In 1998, he started his masters studies in the University of Brasilia, and then went on to do a PhD in Molecular Pharmacology at the University of Brasilia and INSERM-Paris, France. In 2006 he went to the UK to start a post-doc at the MRC-Laboratory of Molecular biology in Cambridge, followed by a post-doc at the University of Leicester, focused on the structural studies of nuclear receptor and chromatin. He is currently a Professor of Pharmacology of the Pharmacy School at the Universidade de Brasilia. He has published several articles in important journals such as Nature, Cell and Trends in Pharmacological Science. In April 2015, he founded Nucleosantos Therapeutics, a start-up that is working on the discovery and development of exogenous Nucleosome-Binding Molecules

Abstract:

Chromatin is the major regulator of gene expression and genome maintenance. Proteins that bind the nucleosome, the repetitive unit of chromatin, and the Histone H4 tail are critical to establish chromatin architecture and phenotypic outcomes. Intriguingly, nucleosome-binding proteins (NBPs) and H4 tail peptide compete for the same binding site at an acidic region on the nucleosome surface. Although the essential facts about the nucleosome were already revealed 17 years ago, new insights into its atomic structure and molecular mechanisms are still emerging. In this talk, I will feature the nucleosome surface as a drug target to control chromatindynamics and, consequently, gene expression and genome maintenance. I will cover the key aspects of chromatin architecture upon binding of protein and exogenous molecules (exogenous Nucleosome Binding Molecules - eNBMs) to the nucleosome. Moreover, I will discuss the impact and development of eNBMs, presenting some of our results in silico, in vitro and in cell-based assays

Biography:

Lars Von Olleschik-Elbheim has completed his PhD in medical microbiology at the age of 33 years from Westfälische Wilhelms-Universität, Münster, Germany. In the past 17 years he has been working for both pharmaceutical and diagnostics companies in various positions. Dealing with the diagnostics and treatment of environmental, diet and behaviour related diseases, his interest became focussed on the effects of personalized nutrition within the frameset of personalized medicine. Within this context he is currently focussing on the effects of Vitamin D and adaptogens, when it comes to stress and age related diseases and anti-ageing in general. The proper use of companion diagnostics for consultation and monitoring is one of his additional topics of interest in regard to personalilized nutrition and anti ageing.

Abstract:

Clinoptilolite is an outstanding microporuous material with a superlarge surface of volcanic rock origin with special selective binding capacities towards heavy metals, small positive charged molecules, and even viruses. Its very interesting, thqt encages mainly the “bad boys” and thus supports their inactivation and depletion from the human body. Used since thousands of years by men and even being part of instincive activities in some animals it was more or less fogotten until the Tschernobyl catastrophy led to intense research activities in order to protect the health of the people as good as possible. Clinoptilolite showed to be the best material to prevent the environment from further intoxication and it is even capable of depleting substances like radioactive Cesium out of the human body. Nowadays there is a rising amount of publications with case reports and clinical studies showing that the “typical diseases of the elderly”, like e.g. diabetes, cancer, ostephorousis, some forms of brain damage can be slowed down or stopped in their progression, sometimes eff ectively treated by the use of clinopotilolite. By blocking toxic compounds from interfering with internal regulatory processes and the capability to deplete them from the human body it shows anti-aging eff ects e.g. on skin, liver, heart, brain, bones, wound healing, hair, and overall endurance. Combined with the right diagnostics clinoptilolite is more and more prooving as a very good part of the medical toolbox

Biography:

Reda Mohamed El badawy has completed her MD at Banha University ,Faculty Medicine. She is worked at King Saud University and King Khaled University Saudi Arabia. She has published more than 25 papers in reputed journals

Abstract:

The relation between Non –Alcoholic liver disease(NAFLD) and cardiovascular diseases (CVD)has not been elucidated with non diabetic obese normal healthy subjects. Th e aim: Was to evaluate the use of simple biomarkers as predictors for NAFLD and consequanly CVD. Seventy seven Subjects divided into two groups: Group( 1) included 37 patients known to have diabetes mellitus type 2 , Group( 2) included 40 healthy subjects who have no medical illness. Results: Th ere was a statistical signifi cant diff erence for all laboratory parameters between the 2 groups except for total bilirubin, direct bilirubin and ALT/AST ratio. In DM group fatty liver was diagnosed in 22/37(54%) by abdominal ultrasound. Th e median values of TG, insulin, leptin, insulin resistant, ALT ,GGT,AST and ALT/AST ratio in diabetic patients were statistically signifi cant higher in those with fatty liver compared with those without fatty liver (P values <0.05). In group 2 fatty liver was diagnosed by abdominal ultrasound in 12/40( 30%). Th e median values of FBS,TG, cholesterol, insulin, leptin, insulin resistant, ALT ,GGT,AST and ALT/AST ratio in the healthy subjects were statistically signifi cant higher in those with fatty liver compared with those without fatty liver (P values <0.05).Th e sensitivity and specifi city of GGT, ALT/AST ratio was as follow 100.%, 59% and 73%, 55% at a cut off value of 45 IU/l and 1.9 respectively. Conclusion: Elevated liver enzymes, although in normal ranges, play a role in early diagnosis of fatty liver disease which has a predict for the presence of cardiovascular diseases especially carotid intimal thickning

Biography:

Guo-Hui Fu has completed her MD from Jiamusi Medical College, China and then completed PhD from Harbin Medical University, China and Post-doctoral studies from Kyushu University School of Medicine, Japan. She is the Dean and Professor of Pathology Center, Shanghai Jiao Tong University School of Medicine. She has published more than 81 papers in reputed journals and has been serving as an Editorial Board Member of repute

Abstract:

Epidermal Growth Factor receptor 2 (HER2) is expressed in 6%-23% gastric cancer tissues. Administration of Trastuzumab, a fully humanized monoclonal antibody targeted to the HER2, is associated with survival rate in HER2-positive gastric cancer (GC) patients. Gastrin is a major gastrointestinal hormone proven to have an inhibitory eff ect on GC in vitro and in vivo. Here, we report the synergistic inhibitory eff ects of Trastuzumab and Gastrin on HER2-negative GC cells through the Gastrin/Cholecystokinin B receptor (CCKBR) pathway. Trastuzumab upregulated CCKBR protein levels but could not initiate its signal transduction, whereas Gastrin increased the levels and activation of CCKBR. Molecular experiments indicated that Trastuzumab and Gastrin co-treatment synergistically enhanced the stability of CCKBR. Moreover, their combined treatment synergistically arrested GC cells at G0/G1 phase, down-regulated levels of GC-related proteins, including anion exchanger (AE) 1, Cyclin D1, β-catenin and Cytoplasmic p16, and promoted nuclear translocation of p16. In addition, combination treatment upregulated AE2 levels, which are reduced in GC tissues. Th e in vivo synergistic anti-GC eff ect of combined treatment was confi rmed in xenograft experiments. Th e results demonstrated the synergistic eff ect of Gastrin and Trastuzumab in the suppression of GC

Biography:

Maysa Kamal Salama has completed her PhD from Cairo University School of Medicine. She was the head of Medical Biochemistry and Molecular Biology Department. She has published more than 30 papers in reputed journals and serving as an Editorial Board Member of repute

Abstract:

To investigate the association of the functional monocyte chemotactic protein-1 (MCP-1) promoter polymorphism (A-2518G) with spontaneous bacterial peritonitis (SBP). Fift y patients with post-hepatitis C liver cirrhosis and ascites were categorized into two groups; group I included 25 patients with SBP and group II included 25 patients free from SBP. In addition, a group of 20 healthy volunteers were included. We assessed the MCP-1 gene polymorphism and gene expression as well as interleukin (IL)-10 levels in both blood and ascitic fl uid. A signifi cant MCP-1 gene polymorphism was detected in groups I and II (P=0.001 and 0.02 respectively). Group I was associated with a signifi cantly higher frequency of AG genotype [control 8 (40%) vs SBP 19 (76.0%), P < 0.001], and group II was associated with a signifi cantly higher frequency of GG genotype when compared to healthy volunteers [control 1 (5%) vs cirrhotic 16 (64%), P < 0.001]. Accordingly, the frequency of G allele was signifi cantly higher in both groups (I and II) [control 10 (25%) vs SBP 27 (54%), P < 0.001 and vs cirrhotic 37 (74.0%), P < 0.001, respectively]. Th e total blood and ascetic fl uid levels of IL-10 and MCP-1 gene expression were signifi cantly higher in group I than in group II. Group I showed signifi cant reductions in the levels of MCP-1 gene expression and IL-10 in the whole blood and ascetic fl uid aft er therapy. MCP-1 GG genotype and G allele may predispose HCV infected patients to a more progressive disease course, while AG genotype may increase the susceptibility to SBP. Patients carrying these genotypes should be under supervision to prevent or restrict further complications.

Biography:

Cardiac metabolism is essential in myocardial contraction. Here, we analyzed the eff ect of metabolic substrates (fatty acids, pyruvate and lactate in normal Tyrode’s solution, termed NF) on myocyte contractility in rat left ventricular myocytes. Our results showed that NF signifi cantly increased myocyte contraction and intracellular Ca2+ transients. L-type Ca2+ current or Na+- Ca2+ exchanger activity was not increased and myofi lament Ca2+ sensitivity was reduced by NF, suggesting key role of myofi lament on cardiac Ca2+ homeostasis and contraction with NF. Furthermore, NF diminished insulin-dependent tyrosine phosphorylations of insulin receptor or receptor substrate and eNOS-Ser1177. Beta-adrenergic stimulation with isoprenaline signifi cantly increased spontaneous myocyte contraction during diastole in NF. Collectively, NF impairs insulin signaling and reduces bioavailability of eNOS-derived NO, which desensitizes myofi lament Ca2+ sensitivity, increases Ca2+ level and contraction. In addition, it predisposes beta-adrenergic arrhythmogenesis in cardiac myocytes. Th e results reveal that it resembles an in vitro model of cardiac metabolic syndrome

Abstract:

Yin Hua Zhang has completed her PhD in 1999 from Seoul National University, College of Medicine and Post-doctoral studies from Oxford University, Cardiovascular Medicine. She directs Cardiovascular Laboratory studying Physiology and Pathology of the heart. She has published more than 40 papers in high ranking journals and is holding a number of national grants

Biography:

Abstract:

Drugs and specifi c supplements used for personalized medicine can be used to manage neurological disorders based on an individual’s personal genomic profi le. Th e development of an improved evidence-base for personalized and patient-decision making about the choice to use a dietary supplement can be a wise decision that provides health benefi ts. Addresses the wide variation in biological response to fi xed-dose supplementation, allow the researchers to advance personalized clinical care by estimating the specifi c supplement dosage needed to achieve the targeted blood concentration. Th e goal is to develop patient-specifi c therapeutic strategies aimed at improving patient health when it comes to getting the right drug and the right supplement to the right patient for optimal results. During the last 40 year, patients with Spielmeyer-Sjögren disease were treated with antioxidant supplementation. Th e patients were supplemented with a combination of vitamin E, vitamin C, methionine and butylated hydroxytoluene (BHT). As the disease began to progress, the treatment was changed to a combination of sodium selenite and vitamin E in some patients. Th e same combination was also given to children (vitamins B2 and B6 were also added for several years.) who had not received previous antioxidant supplementation. Th e latter combination (called the Westermarck formula) appeared to be helpful to some patients. Statistical correlations between various neurological items and relevant laboratory data were sought. In the older patients a signifi cant correlation was found between neurological dysfunction and ceruloplasmin, and also between epilepsy and ceruloplasmin, while a negative correlation was noticed between neurological dysfunction and glutathione peroxidase. In the younger patients, a negative correlation was observed between superoxide dismutase and epilepsy. Serum apolipoprotein B levels were below the normal range in the 6 patients investigated. So far the Westermarck formula seems to have been the best treatment devised yet in Spielmeyer-Sjögren disease, but further studies are needed for a better understanding of the pathogenesis of neuronal ceroid-lipofuscinoses disorders. For treating various health problems, it makes sense to take into account diet, genetic makeup, pathogens, pollutants and environment exposure, and to then judiciously use supplements to counter any nutrient defi ciencies. Making dietary changes alone frequently helps correct some of the most common. A good diet is a foundation but, for optimum health, we need to look aft er all aspects of being.